CONFOCALMICROSCOPY Archives

July 2001

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Subject:
Re: Histological Dyes and fluorescence
From:
Michael Cammer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 26 Jul 2001 11:37:34 -0700
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We have found that tol blue, DAB, silver enhanced gold, and lead ATPase all
block or quench fluorescence.


>> Most transmitted light histological dyes seem to have a lot of
>> fluorescence to them.  Could someone suggest a stain/dye that works well
>> in transmitted light, but adds little fluorescence background to the
>> sample.
>try toluidine blue 01%

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
(718) 430-2890       Fax:  430-8996      URL:  http://www.aecom.yu.edu/aif/

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