CONFOCALMICROSCOPY Archives

November 1998

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
RE permeabilization of whole mounts
From:
D Armstrong <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sat, 14 Nov 1998 11:37:46 +0000
Content-Type:
TEXT/PLAIN
Parts/Attachments:
TEXT/PLAIN (17 lines) 
Hi,

We use 1 x PBS, 1% Triton X-100, 1% BSA as a buffer for all steps after 4%
Paraformaldehyde fixation in whole Drosophila brains. This works fine with
anti-betagalactocidase but might not be suitable for other antibodies.

I've also used 0.1% Tween 20 some time ago and it appeared to work fine.

As for using alcholos/xylene, its fine so long as there are no tissues
connected that have pigments in them (we have problems with fluorescent
pigments in the Drosophila retina).

Cheers

Douglas Armstrong
University of Glasgow.

ATOM RSS1 RSS2