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Hi 

GCaMP3 is very bright and takes about 1-2 weeks for it to reach full expression 
potential. YC3.6 is not as bright but doesn't have detrimental effects on the cells 
for about 2 months. GCaMP3 will start making cells sick within 3 weeks. It's very 
much density dependent so combining the dye with an agent that increases the 
spread will help a lot. Also, injecting at a slower rate (~.5um/min) increases 
spread greatly. 

DRoumis