Dear Olivia, 
Improvision is very good though it is costly, and the Roper's cascade 512B or Hamamatsu back-illuminated CCD cameras would be of great help, especially if you are interested to detect smaller number of rather functional molecules, not the aggregates, inclusions or insoluble precipitates within the cell.
Cell Biology/Confocal Microscopy is known of its numerous artifacts, especially upon protein overexpression.
 
Best regards,
 
Vitaly
NCI-NIH
HIV-DRP 

	-----Original Message----- 
	From: Confocal Microscopy List on behalf of Steele-Mortimer, Olivia (NIH/NIAID) 
	Sent: Tue 3/9/2004 5:23 PM 
	To: [log in to unmask] 
	Cc: 
	Subject: Re: Spinning Disc Confocal
	
	

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	Thanks for all your suggestions. We already have the PE system (not new RS)
	and I know that technically both Metamorph and Openlab can run all the
	components. We just have to decide which one to go for. Our experience with
	the PE software has been frustrating to say the least. We want something
	stable and relatively user friendly. We already have Meta on a couple of
	other systems but  are very impressed with some other software from
	Improvision (Volocity). We also have a lot of Mac users so a Mac system
	would be the popular choice.
	Olivia
	
	Olivia Steele-Mortimer
	Principal Investigator
	Laboratory of Intracellular Parasites
	NIAID, NIH
	Rocky Mountain Laboratories
	903 South 4th, Hamilton, MT 59840
	
	Tel: 406-363 9292      Fax: 406-363 9380