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Dear group members,

I have a very simple question that I require help with. Unfortunately our confocal expert has 
been booked off for three months and I am struggling.

I am using a Zeiss LSM 510 Meta. I am viewing bacteria using a 100 x oil immersion 
objective. I have stained my cells with Nile Red or Draq 7 and I am using the crop function 
to get  suitable images of single cells.

I am planning to publish my work, but I have no idea how to obtain a DIC image or suitable 
image that will allow me overlay my flourescent image of single cells. Is it possible to use 
the crop/zoom function of the software to obtain a DIC image of the same cell at the same 
magnification? 

Thank you in advance