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Hi-
We have a Noran confocal which I've been using to generate time-series.
The computer we have the analysis software on is dying a long painful
death.  We will be out of luck when it finally gives up because no more
licenses can be generated to my knowledge.  I can save the data as a
numbered series of tiffs.  The problem is that I have thousands of tiffs
per experiment in many cases.  I don't want to do anything exotic with
the data, just brightness over time for different regions of interest.
I thought I could use Scion/NIHImage to do this, but I'm having problems
opening the tiffs as a stack.  Furthermore, the limit according to the
documentation I've read is 400 images since it's run entirely in RAM.
Are there any alternatives (cheap preferably) or workarounds that anyone
can suggest for my very large files?
Thanks in advance-
Sarah
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Sarah Locknar, Ph.D. 
Director, Neuroscience COBRE Imaging / Physiology Core 
College of Medicine, University of Vermont 
E015 Given Building 
89 Beaumont Ave. 
Burlington, VT 05405 
802-656-0413 
802-656-8704 (fax) 
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