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November 2008

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Subject:
Image analysis
From:
"Anchall ............" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 14 Nov 2008 03:56:55 +0100
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Hello all,
           This query is regarding image analysis.I am lookin for some way I
can quantify the following data.
I have images with a protein A usually localised in ER and Golgi.But when I
overexpress another protein B which kind of solubilises PROTEIN A then I see
diffuse expression of protein A all over the cell basically in cytoplasm.Now
what I want to find out is whether the protein is still present in ER &
Golgi but it is not so distinguisable as now there is lots in cytoplasm
too.Is there any way I can quantify this.. using IMAGE J as i mostly use
only that...
I hope some help in this regard..
Thanks
Anchal

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