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Julie,

Your approach sounds reasonable.  For details on volume density and other
stereological issues, suggest you either contact John Russ (post the
question to the MSA listserver and he will see it) or to my colleague, Dr.
Barry Fookes (I've cc'd him on this message).  He's a real wizard for this
sort of thing.

Good hunting!
  Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931  FX: 413-746-9311  Web: www.MicroscopyEducation.com


"Why didn't they teach us that sooner?"  ... probably because no one
thought to call MME about customized, on-site courses.  Offered in all
areas of microscopy, sample prep,and image analysis, they make an immediate
impact on your productivity.
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At 08:57 AM 4/3/03 -0600, Julie A Reynolds wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>
>I am interested in estimating the volume density of mitochondria in brine
>shrimp embryos. The shell around the encysted embryos makes it difficult to
>fix the embryos for EM, so I am looking for alternative methods. Somebody
>in my lab suggested that I squish the embryos, label the mitochondria with
>MitoTracker and use a point counting method to estimate # of mitochondria
>in images taken with a confocal microscope.
>
>Has anyone else tried anything remotely like this? If so do you have any
>words of wisdom? So far I am having only limited success with this
>technique.
>
>
>Any assistance will be greatly appreciated.
>
>Julie
>
>
>Julie Reynolds
>Graduate Research Assistant
>Dept. of Biological Sciences
>Louisiana State University
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