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Sounds interesting - please do send me a copy.

But my question is why is this necessary?  You have absolute
standards built in the in the form of your CW lasers (6 lines
on our system) - their position shows how accurately your system
is calibrated and the FWHM shows the resolution.  What more do
you need?

                                                     Guy



Quoting Robert Zucker <[log in to unmask]>:
>
> At the recent microscopy meetings (MM2004) in Savannah a paper by R
> Zucker and J. Lerner was presented entitled  “Confocal Microscopy System
> Performance: Foundations for Measurements, Quantitation and Spectroscopy
> ”
>
> This Confocal QA spectroscopy test was developed to measure spectral
> performance and to serve as guidelines for investigators to assess both
> the performance of their instruments as well as the quality of their
> data. This spectral characterization test is well suited to all
> wavelength dispersive CLSM systems including the Leica SP series (SP),
> the Zeiss LSM510 Meta (Meta) and Olympus FV1000 confocal microscopes.
> We used an inexpensive, eye-safe, battery operated, multi-ion discharge
> lamp (MIDL) (LightForm, Inc., Hillsborough NJ) containing mercury ions
> and inorganic fluorophores as an absolute reference light source because
> it emits stable, reproducible, peaks between 400 and 650 nm.  It is
> worth applying this test to your systems.
>
> An abstract describing this presentation has been published and is
> available on request.
>
> Bob
>
>
> Robert M. Zucker, PhD
> U.S. Environmental Protection Agency
> Office of Research and Development
> National Health and Environmental Effects Research Laboratory
> Reproductive Toxicology Division, MD 72
> Research Triangle Park, North Carolina, 27711
> Tel: 919-541-1585; fax 919-541-4017
> e-mail: [log in to unmask]


--
Associate Professor Guy Cox
Electron Microscope Unit, F09
University of Sydney NSW 2006
+61 2 9351 3176