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May 2014

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From:
Neil Anthony <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 16 May 2014 10:26:15 -0400
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*****
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Emmanuel, I'm not sure if it's one of the latest options or not, but has 
anybody looked at the optiMOS from QImaging?

It has a CIS1920F sCMOS sensor, and everything else looks as good as the 
others but with a reduced sensor size (1920x1080).  It's less that 10k, 
so it's considerably cheaper.  They don't mention the shutter type, but 
it has to be a rolling shutter, right?

Also, there's the Flash 4.0 LT.  I think it's closer to 12k instead of 
the 16-20k of the fastest and global shutter versions.  It's USB 3.0 
which makes life a little easier, and it's got a better QE and less 
noise than the cheaper version of the Zyla 5.5 (which does offer 30% 
more real estate; 2561x2160 vs. 2048x2048 both with 6.5µm pixels).

Does anybody know of a webpage/document out there that has all the 
details and prices in one place?  It would be great to condense all 
these things.

Neil


On 4/19/2014 12:22 PM, Emmanuel Levy wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> We intend to buy a spinning disk confocal microscope and we are therefore
> looking for the best camera to suit our need.
>
> The main use will be imaging of fluorescently tagged proteins expressed in
> live cells.
>
> One thing is certain, we will buy a sCMOS, but we are unsure of which one
> is best.
>
> For a while, it is my understanding that the Hamamatsu Flash4 V2 was the
> best - but couple of month ago the same chip has been used in the Zyla 4.2
> (Andor) and in the PCO Edge 4.2.
>
> All of them exhibit highly similar characteristics, with QE ~70%, low read
> noise, 100fps with full chip, etc.
>
> I guess the only way to see what exactly are the advantages/drawbacks would
> be to use them in the same setup and compare them side by side.
> Unfortunately I cannot do that and therefore I'd be happy to hear about any
> experience you have.
>
> I insist that only the late models should be compared (released in the fall
> of 2013 I believe). If you have had a bad experience with previous models
> (e.g., Andor Neo), it does not apply here.
>
> Thanks in advance,
>
> Emmanuel
>

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