CONFOCALMICROSCOPY Archives

January 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
C. elegans fixation, fluorescent proteins
From:
Guenter Giese <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 18 Jan 2001 16:40:22 +0100
Content-Type:
text/plain
Parts/Attachments:
text/plain (26 lines) 
Dear colleagues,

some months ago the question concerning C. elegans nematodes for light
microscopy was raised. Microwave and chemical fixation
(acrolein/formaldehyde/glutaraldehyde) were suggested.

Can anyone provide a protocol or reference (or contact) for fixation
preserving GFP (YFP, etc.) fluorescence?


Cheers,

Guenter

----------------------------------
Dr. Guenter Giese
MPI fuer Medizinische Forschung
Dept. of Biomedical Optics
Jahnstr. 29
D-69120 Heidelberg
Phone (Germany or 0-)6221-486-360
Fax   (Germany or 0-)6221-486-325
e-mail: [log in to unmask]
http://wbmo.mpimf-heidelberg.mpg.de/Biomedizinische.Optik.html
------------------------------------------

ATOM RSS1 RSS2