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Hi Robert,
Thanks a lot for your answer. Than I have a "reverse" question. Can a few mercury lamp high voltage power supply (2kV) have an influence on the antenna or relay? And at which distance apart it can be safe? Thanks in advance for your answer.
Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
tél. (514) 343-6111 poste 5148
________________________________
De : Confocal Microscopy List [mailto:[log in to unmask]] De la part de Robert Atkinson
Envoyé : 22 novembre 2006 03:44
À : [log in to unmask]
Objet : Re: Net Wireless antenna interferences?
Hi Monique,
I'm a lurker on this list, but this is one question I can answer - I'm a radio engineer.
While there is a possibility of interference between any electronic equipment, there should be no problem with properly designed professional equipment. The power transmitted from a wireless network access point is quite low, much less than a mobile phone. I would not expect problems unless it was setup right next to the microscope.
If you do suspect interference, it's easy to check if the network access point is at fault, just turn it off. If it's at fault the interference will stop.
Hope this helps,
Robert Atkinson.
________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Vasseur Monique
Sent: 21 November 2006 15:50
To: [log in to unmask]
Subject: Net Wireless antenna interferences?
Hi all,
I don't know much about net connections and communication technologies. In our department, there is a project of installing a 10 megabyte antenna and/or a relay in the microscopy room or a few meters from it, so that students can connect their laptop to the local net without the need of cable connection. This is probably a stupid question but I am wondering if this is generating a kind of waves that could interfere with the output of CCD cameras. Any comments are welcome.
Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
tél. (514) 343-6111 poste 5148
________________________________
De : Confocal Microscopy List [mailto:[log in to unmask]] De la part de Bill Miller
Envoyé : 21 novembre 2006 10:11
À : [log in to unmask]
Objet : Re: food and CLSM
Confocal Raman Microscopy certainly has wide spread application in food science. WITec has a new Application note on Food Analysis on their web site - http://www.witec.de/download/raman_01.html
Bill Miller
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Umberto
As well as the "high end" techniques of multiphoton confocal, specific labelling and SEM, you may consider basic bright field light microscopy. It depends what you want to look for but I have seen several papers where speculations were made about ingredient locations based solely on SEM, whilst a good old fashioned light microscope would have revealed starch, protein, air bubbles etc. I have used a dilute I/KI solution to stain starch and fast green to stain protein.
You can find out more about light microscopy of food materials in Olga Flint's Royal Microscopical Society Handbook 30: "Food Microscopy" published by Bios. There may be helpful sample preparation techniques for cereals that you could adapt to the confocal. I do feel that good old fashioned bright field or polarized light microscopy is a much neglected technique in food studies.
Re: confocal techniques - you may be interested in this paper
Auty, M.A.E., Twomey, M., Guinee, T.P. and Mulvihill, D.M. 2001. Development and applications of confocal scanning laser microscopy methods for studying the distribution of fat and protein in selected food products. Journal of Dairy Research. 68: 417-427.
These are general methods for dairy products and don't distinguish between different proteins or specific lipids. You will probably have to develop a method yourself for this. A problem I have found with foods is that of immobilizing the substrate. Paraffin or resin embedding is one approach. Most published data on specific labeling of food ingredients used pre-labelled proteins (antibody-based) or polysaccharides (covalent labeling) then mixed them in a model system. I'd be interested to hear of anyone who has localized specific proteins/polysaccharides/lipids in any heterogenous food system.
Regards
Mark
Dr. Mark Auty
Food Processing & Functionality Department
Moorepark Food Research Centre
Teagasc
Moorepark
Fermoy, Co. Cork, Ireland
tel: +353 25 42442
fax: +353 25 42340
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From: Confocal Microscopy List [ mailto:[log in to unmask] <mailto:[log in to unmask]> ] On Behalf Of Umberto Fascio
Sent: 20 November 2006 09:59
To: [log in to unmask]
Subject: food and CLSM
Dear all
I'm a food technologist and my field of research is Cereal Science and Technology. In the recent years I've approached to Scanning Electron Microscopy and I've found it very fascinating and really useful to my research. I'd like to go in depth with the knowledge of my materials by using the Confocal Laser Scanning Microscopy: I had just a short "meeting" with it, but it was enough to catch me!!! Well, in my research I deal with kernels, flours and baking processes and I'd like to better study, also from a microstuctural point of view, these materials and the interaction between protein-starch-fiber-lipids when the flour is mixed to give dough ... but I absolutely ignore how to treat them (sample preparation, specific fluorescent labels...) for the CLSM observation. Please, would be so kind to give me any help?
Many thanks in advance,
Manuela
Dr. Manuela Mariotti
DiSTAM - Food Technology
University of Milan
via G. Celoria, 2
20133 Milan, Italy
phone: +39.0250316625
fax: +39. 0250316632
e-mail: [log in to unmask]
Dott. Umberto Fascio
C.I.M.A.
Centro Interdipartimentale Microscopia Avanzata
Via Celoria 26 - 20133 Milano
tel: +39 0250314807/14876
fax:+39 0250314802
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