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What would happen?  Generic answer...poor images.  You would still have
"something" in between the coverslip and the glass slide, Air, with its
resultant refractive index mismatch between the two pieces of glass.  It
would be much preferable to have mounting medium of some kind, depending
on your sample, ideally with a refractive index close to glass.  

What is your sample, and what are you trying to image?

--
Samuel A. Connell                                        
Director of Light Microscopy
Cell & Tissue Imaging Center
St. Jude Children's Research Hospital
332 North Lauderdale St., E7061
Memphis, TN 38105-2794
(901) 495-2536 
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Oky
Sent: Thursday, November 08, 2007 12:33 PM
To: [log in to unmask]
Subject: Imaging without medium?. .

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Dear all,

I am wondering what happen if I don't use any medium between cover and
slide glasses. No water or anything in between.

Thank you for your response in advance.


-- 
Ohkyung Kwon, Ph D

Postdoctoral Research Associate
Sustainable Engineered Materials Institute
Virginia Tech