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October 2014

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Subject:
Methanol fixation for primary cultures neurons.
From:
nikhil pandya <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 22 Oct 2014 21:42:03 +0200
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Greetings!
A bit of Neuroscience specific question.

I have been testing antibodies for Post synaptic density proteins like
PSD95 and AMPA and NMDA receptor in primary hippocampal neurons. I am
finding it difficult to get good labelling in the PSD. Is it recommended to
use methanol to fix and stain PSD proteins in general? Are there any
disadvantages to using methanol instead of PFA fixation?

-- 
Nikhil Janak Pandya.
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