Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I've been thinking about building a system like that ever since one of our
users started doing overnight recordings and his samples sometimes drift
vertically and thus would need an autofocus system to correct for that.  If
you could tap into the Z-controller circuit of your system, you would be
able to drive that with a signal that's proportional to the vertical drift.
The drift could be scaled with e.g. calculating average intensity during a
Z-stack from a certain area of the image that's fairly thin.  Similar logic
would work for the autoexposure as well.  Want to try it?

Zoltan

On Dec 7, 2007 4:41 PM, Nuno Moreno <[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Autoexpose will bleach everything, right?
>
> Regarding the adaptative focus that I mentioned before, there are
> commercial system that with minimum light and before an acquisition
> "measure" the cell position and adapt the focus. But this is an half
> adaptation. It could be that it does not need to readjust the focus.
>
> What I was counting with would be after the acquisition, if it is out of
> focus, it make the adjustment base in some kind of sensitivity
> parameter. This could be after 10 time points but it might be that it
> would never need such adjustment.
>
>
> About the intensity variations I'm not talking about post processing
> adjustments. If it gets saturated there are no post processing that can
> help you.
>
> Regards,
> NM
>
>
>
>
>
> Shalin Mehta wrote:
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Nuno,
> >
> >  Wouldn't auto-exposure on cameras suffice for maintaining constant
> > intensity?
> >
> > Apparently most of the commercial adaptive optics systems are geared
> > towards astronomy. Perhaps you have known this already:
> > http://cfao.ucolick.org/
> > Interesting to note that James Webb space telescope will have hardware
> > and intelligence for adaptive optics evolved from algorithms developed
> > for correcting aberrations for hubble telescope.
> >
> > Regards,
> > Shalin
> >
> >
> > On Dec 7, 2007 10:43 PM, Nuno Moreno <[log in to unmask]
> > <mailto:[log in to unmask]>> wrote:
> >
> >     Search the CONFOCAL archive at
> >     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >     Does anyone knows any commercial widefield SYSTEM that makes an
> >     adaptative focus. And I mean adaptative (follows the cell.
> >
> >     The other feature is a commercial system that keeps intensities, i.e
> .,
> >     if you have something with different protein expression levels over
> >     time, the system will correct the exposure time so that at the end
> the
> >     intensities are constant.
> >
> >     Many thanks,
> >     --
> >     Nuno Moreno
> >     Cell Imaging Unit
> >     Instituto Gulbenkian de Ciência
> >     http://uic.igc.gulbekian.pt <http://uic.igc.gulbekian.pt>
> >     http://www.igc.gulbekian.pt
> >     phone +351 214464606
> >     fax   +351 214407970
> >
> >
> >
> >
> > --
> > ~~~~~~~~~~~~~~~~~~~~~~~~~
> > Shalin Mehta
> > Graduate Student in Bioengineering, NUS
> > mobile: +65-90694182
> > blog: shalin.wordpress.com <http://shalin.wordpress.com>
> > ~~~~~~~~~~~~~~~~~~~~~~~~~~
>
> --
>  Nuno Moreno
> Cell Imaging Unit
> Instituto Gulbenkian de Ciência
> http://uic.igc.gulbekian.pt
> http://www.igc.gulbekian.pt
> phone +351 214464606
> fax   +351 214407970
>



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Zoltan Cseresnyes
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Dept. of Zoology University of Cambridge
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