CONFOCALMICROSCOPY Archives

September 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: Pinhole size
From:
Weijun Qian <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 5 Sep 2001 13:52:12 -0700
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According to the orgininal design of Roger Tsien, the adjustable confocal
aperture size is from 0.53 to 17 microns at the specimen plane.  But I am
not sure about the exact size of Nikon RCM.  I will be interested to know
the results you will get.  Good luck!

Weijun Qian
Department of Chemistry
University of Florida
Gainesville FL 32611
[log in to unmask]


"habo j. jongsma" <[log in to unmask]> wrote in message news:
<[log in to unmask]>...
> Does anyone know the pinhole sizes of the Nikon RCM 8000 confocal
> microscope.The manual only tells that there are two : a large one and a
> small one; to measure or calculate  PSFs I would need the actual sizes.
> Thanks a lot,
>
>
> Habo J. Jongsma, PhD
> Department of Medical Physiology
> University Medical Center Utrecht
> PO Box 85060
> 3508 AB Utrecht
> The Netherlands
> tel: +31-30-2538900
> fax: +31-30-2539036
>

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