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Industry Response:
There absolutely is a 514 DPSS available from 20 mW up to 150 mW available
in the Sapphire form factor from Coherent. We use this laser, often in
conjunction with the 445 CUBE in our LaserStack for CFP/YFP (and their
newer brothers and sisters in FP evolution) imaging for FRET or otherwise.
------------------------
Samuel A. Connell
Sales Manager
Pacific Region-North America
Intelligent Imaging Innovations, Inc
3250 Ocean Park Blvd, Suite 202
Santa Monica, CA 90405
Cell: (858) 692-4510
[log in to unmask]
On Wed, Nov 9, 2011 at 4:21 PM, Damir Sudar <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> See: http://www.cobolt.se/**coboltfandango515nm.html?**
> gclid=CPCOueXlqqwCFQyEhwodQn-**aAw<http://www.cobolt.se/coboltfandango515nm.html?gclid=CPCOueXlqqwCFQyEhwodQn-aAw>
>
> No connection, just considering about switching away from our old Ar Ion
> as well.
>
> - Damir
>
>
> On 11/9/2011 3:36 PM, Guy Cox wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Are you sure there is a 514nm DPSS? I think that wavelength would have
>> to be an OPS and I don't know anyone who makes one. Coherent do make a
>> 505nm OPS which is intended to stand in for both the 488& 514 lines of
>>
>> an Argon laser, and I rather suspect that this could be exactly what you
>> need. But I have no experience of it. (I do have a Coherent 488nm
>> OPS).
>>
>> Guy
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox CRC Press / Taylor& Francis
>>
>> http://www.guycox.com/optical.**htm<http://www.guycox.com/optical.htm>
>> ______________________________**________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Australian Centre for Microscopy& Microanalysis,
>>
>> Madsen Building F09, University of Sydney, NSW 2006
>>
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________**________________
>> http://www.guycox.net
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY@**LISTS.UMN.EDU<[log in to unmask]>
>> ]
>> On Behalf Of Tim Feinstein
>> Sent: Thursday, 10 November 2011 7:34 AM
>> To: [log in to unmask]**EDU <[log in to unmask]>
>> Subject: FRET in the time of DPSS
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Hello all,
>>
>> We want to spec a four-laser launch for a new live cell system that will
>> handle both CFP/YFP FRET and red/green imaging. However, I am sad to
>> see that gas lasers are no longer speccable and so the freebie 514 laser
>> line is gone. We would therefore have to spec a 514 DPSS and forego the
>> far-red line.
>>
>> I was wondering whether there is a way to do more (or at least the same)
>> with less. 488 nm excites YFP well enough, so in theory I could image
>> CFP/YFP using a scan head dichroic with cutouts for 442 and 488 nm laser
>> lines. In my experience 442 nm laser excitation (via TIRF) causes
>> negligible YFP excitation and 488 nm does not excite CFP, so it is
>> possible that I could gain speed by passing everything through a single
>> broad bandpass filter (e.g., 455-550 nm) and alternate excitations.
>> Assuming that cross-talk is not a problem, the most significant cost
>> would be that I lose a decent chunk of CFP emission to the scan head
>> dichroic, but in return I gain a 641 nm laser.
>>
>> Has anyone tried this? Any feedback on or off-list would be much
>> appreciated.
>>
>> Thanks and all the best,
>>
>>
>> TF
>>
>> Timothy Feinstein, PhD
>> Postdoctoral Fellow
>> Laboratory for GPCR Biology
>> Dept. of Pharmacology& Chemical Biology
>>
>> University of Pittsburgh, School of Medicine
>> BST W1301, 200 Lothrop St.
>> Pittsburgh, PA 15261
>>
>> -----
>> No virus found in this message.
>> Checked by AVG - www.avg.com
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>>
>
> --
> Damir Sudar - Staff Scientist and Deputy for Technology
> Lawrence Berkeley Laboratory / Life Sciences Division
> One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
> T: 510/486-5346 - F: 510/486-5586 - E: [log in to unmask]
> WWW: http://www.lbl.gov/lsd/People_**&_Organization/Scientific_**
> Staff_Directory/Sudar_Lab.html<http://www.lbl.gov/lsd/People_&_Organization/Scientific_Staff_Directory/Sudar_Lab.html>
>
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