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To add to this, the new 520nm diode lasers are of the first type Guy talks
about. I agree that their center wavelengths can be slightly variable, but
a good vendor will tell you exactly what wavelength is coming out of the
laser you buy. Also, these diode lasers can be modulated electrically so
you can use them without AOM devices.
Craig
On Fri, Nov 11, 2011 at 5:44 AM, Guy Cox <[log in to unmask]> wrote:
> A simple diode laser is a pure semiconductor device. The laser cavity
> is contained within a single chip (basically an LED with mirror layers
> above and below it). Wavelengths currently available are from near UV
> (~365nm) through violet to blue (~470nm), and then down in the red from
> ~640nm. Because the laser cavity is so small the wavelength is not very
> precise, which can be a problem for AOD based devices, though it is not
> likely to be a biological problem. Your 405 and 440 nm lasers are most
> likely simple diodes, as well and your 647nm. If you have a red laser
> pointer this will be a simple diode.
>
> Correction from laser companies welcome, but I hope this helps.
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Australian Centre for Microscopy & Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Craig Brideau
> Sent: Friday, 11 November 2011 12:05 PM
> To: [log in to unmask]
> Subject: Re: FRET in the time of DPSS
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Direct diode means that the laser has a single element. It tends to be
> the
> simplest type of laser; basically it is a big laser pointer.
> Diode-pumped
> solid state use an infrared diode (typically) which pumps some secondary
> medium that gives you your desired wavelength. They are more complex
> than
> direct diode since you now have two separate objects that have to be
> reliably optically coupled. Until recently direct diode lasers have
> only
> been capable of near-IR, Red, and Violet or Blue lines, but a recent
> breakthrough in laser diode chemistry has allowed them to work at around
> 520nm.
>
> Here's an old article from 2009 mentioning one of the first green direct
> diode lasers:
>
> http://displaydaily.com/2009/07/21/green-laser-diode-demonstrated-a-brea
> kthrough/
>
> The current problem is pushing them to longer wavelengths. Projector
> companies want to get them out to 530, 540nm for making displays and
> video
> projectors:
>
> http://www.qmed.com/mpmn/article/suppliers-chip-based-diode-makes-waves-
> green-laser-world
>
> But 'shorter' direct diode green lasers in the 520nm or less range are
> fairly available right now.
>
> Craig
>
>
>
> On Thu, Nov 10, 2011 at 5:09 PM, Jen Clarke
> <[log in to unmask]
> > wrote:
>
> > Hi all
> >
> > This discussion is very timely for our facility, as we are looking to
> > purchase a solide state laser in the ~500-520nm range as soon as
> possible
> >
> > I am insufficiently familiar with the pros and cons of choosing a DPSS
> > versus a OPS or a direct-diode laser
> >
> > From the discussion so far it sounds like the choices for a solid
> state
> > laser for imaging YFP are 515nm DPSS from Spectra-Physics, a 505nm
> Sapphire
> > OPS, a 514nm Sapphire OPS or a 520 direct diode.
> >
> > Guy - I presume by "direct diode" you mean a diode pumped solid state,
> > which I understand would be not as good as a DPSS (please correct me
> if I
> > am wrong)
> >
> > I dont have any idea how an OPS compares to a DPSS laser
> >
> > Any advice as to which of these options might be "best" as an add on
> to a
> > new Olympus FV1000 for the primary purpose of imaging and bleaching
> YFP in
> > the CFP/YFP FRET pair would be greatly appreciated.
> >
> > Kind regards
> > Jen
> > --
> > Jennifer Clarke BSc (Hons) PhD
> > Research Associate, Anatomy and Histology
> > Centre for Neuroscience, School of Medicine
> > &
> > Facility Manager, Optical Microscopy Suite, Flinders Microscopy
> >
> > Flinders University
> > GPO Box 2100, Adelaide 5001
> > Phone: 61 8 8204 6454/ 61 8 8204 6637
> > Email: [log in to unmask]
> >
> >
> > ________________________________________
> > From: Confocal Microscopy List [[log in to unmask]] On
> > Behalf Of Craig Brideau [[log in to unmask]]
> > Sent: Friday, 11 November 2011 5:03 AM
> > To: [log in to unmask]
> > Subject: Re: FRET in the time of DPSS
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > There are also the new direct diode lasers at 520nm if you can handle
> a 6nm
> > red wavelength shift from 514. They've only been around for about a
> year
> > but many manufacturers have lumped them in with their direct-diode
> product
> > lines and they look pretty decent.
> >
> > Craig
> >
> >
> >
> > On Thu, Nov 10, 2011 at 6:08 AM, Tim Feinstein <[log in to unmask]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Hi guys (and Guy),
> > >
> > > Many thanks for the feedback. I like the 505 laser idea in
> particular.
> > > As long as it excites 488 fluorophores well enough it could be
> exactly
> > > what I need. Does anybody have experience with using that in place
> of a
> > > 488 line?
> > >
> > > All the best,
> > >
> > >
> > > Tim
> > >
> > > Sent from my iPad
> > >
> > > On Nov 10, 2011, at 1:57 AM, Guy Cox <[log in to unmask]> wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > *****
> > > >
> > > > The Sapphire series lasers are OPS (Optically Pumped
> Semiconductor) not
> > > > DPSS but, you are right, they do indeed have a 514nm version. I
> don't
> > > > know why I didn't find it, but did find the 505, when I searched
> this
> > > > morning. I still think that the 505 might be exactly what Timothy
> > > > needs, though, since he doesn't want to buy an extra laser.
> > > >
> > > > I have also been contacted off-list and told that Spectra-Physics
> have
> > a
> > > > 515nm DPSS which is close enough to make no difference. I've no
> idea
> > > > what the lasing crystal is.
> > > >
> > > > Guy
> > > >
> > > > Optical Imaging Techniques in Cell Biology
> > > > by Guy Cox CRC Press / Taylor & Francis
> > > > http://www.guycox.com/optical.htm
> > > > ______________________________________________
> > > > Associate Professor Guy Cox, MA, DPhil(Oxon)
> > > > Australian Centre for Microscopy & Microanalysis,
> > > > Madsen Building F09, University of Sydney, NSW 2006
> > > >
> > > > Phone +61 2 9351 3176 Fax +61 2 9351 7682
> > > > Mobile 0413 281 861
> > > > ______________________________________________
> > > > http://www.guycox.net
> > > >
> > > >
> > > >
> > > > -----Original Message-----
> > > > From: Confocal Microscopy List [mailto:
> > [log in to unmask]]
> > > > On Behalf Of samuel connell
> > > > Sent: Thursday, 10 November 2011 1:57 PM
> > > > To: [log in to unmask]
> > > > Subject: Re: FRET in the time of DPSS
> > > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > *****
> > > >
> > > > Industry Response:
> > > >
> > > > There absolutely is a 514 DPSS available from 20 mW up to 150 mW
> > > > available
> > > > in the Sapphire form factor from Coherent. We use this laser,
> often in
> > > > conjunction with the 445 CUBE in our LaserStack for CFP/YFP (and
> their
> > > > newer brothers and sisters in FP evolution) imaging for FRET or
> > > > otherwise.
> > > >
> > > > ------------------------
> > > > Samuel A. Connell
> > > > Sales Manager
> > > > Pacific Region-North America
> > > > Intelligent Imaging Innovations, Inc
> > > > 3250 Ocean Park Blvd, Suite 202
> > > > Santa Monica, CA 90405
> > > > Cell: (858) 692-4510
> > > > [log in to unmask]
> > > >
> > > >
> > > > On Wed, Nov 9, 2011 at 4:21 PM, Damir Sudar <[log in to unmask]>
> wrote:
> > > >
> > > >> *****
> > > >> To join, leave or search the confocal microscopy listserv, go to:
> > > >>
> > > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> > http://lists.umn
> > > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > > >> *****
> > > >>
> > > >> See: http://www.cobolt.se/**coboltfandango515nm.html?**
> > > >>
> > > > gclid=CPCOueXlqqwCFQyEhwodQn-**aAw<
> > http://www.cobolt.se/coboltfandango51
> > > > 5nm.html?gclid=CPCOueXlqqwCFQyEhwodQn-aAw>
> > > >>
> > > >> No connection, just considering about switching away from our old
> Ar
> > > > Ion
> > > >> as well.
> > > >>
> > > >> - Damir
> > > >>
> > > >>
> > > >> On 11/9/2011 3:36 PM, Guy Cox wrote:
> > > >>
> > > >>> *****
> > > >>> To join, leave or search the confocal microscopy listserv, go
> to:
> > > >>>
> > > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> > http://lists.umn
> > > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > > >>> *****
> > > >>>
> > > >>> Are you sure there is a 514nm DPSS? I think that wavelength
> would
> > > > have
> > > >>> to be an OPS and I don't know anyone who makes one. Coherent do
> make
> > > > a
> > > >>> 505nm OPS which is intended to stand in for both the 488& 514
> lines
> > > > of
> > > >>>
> > > >>> an Argon laser, and I rather suspect that this could be exactly
> what
> > > > you
> > > >>> need. But I have no experience of it. (I do have a Coherent
> 488nm
> > > >>> OPS).
> > > >>>
> > > >>> Guy
> > > >>>
> > > >>> Optical Imaging Techniques in Cell Biology
> > > >>> by Guy Cox CRC Press / Taylor& Francis
> > > >>>
> > > >>>
> > > >
> http://www.guycox.com/optical.**htm<http://www.guycox.com/optical.htm>
> > > >>> ______________________________**________________
> > > >>> Associate Professor Guy Cox, MA, DPhil(Oxon)
> > > >>> Australian Centre for Microscopy& Microanalysis,
> > > >>>
> > > >>> Madsen Building F09, University of Sydney, NSW 2006
> > > >>>
> > > >>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> > > >>> Mobile 0413 281 861
> > > >>> ______________________________**________________
> > > >>> http://www.guycox.net
> > > >>>
> > > >>>
> > > >>>
> > > >>> -----Original Message-----
> > > >>> From: Confocal Microscopy List
> > > > [mailto:CONFOCALMICROSCOPY@**LISTS.UMN.EDU
> > <[log in to unmask]
> > > > EDU>
> > > >>> ]
> > > >>> On Behalf Of Tim Feinstein
> > > >>> Sent: Thursday, 10 November 2011 7:34 AM
> > > >>> To: [log in to unmask]**EDU
> > > > <[log in to unmask]>
> > > >>> Subject: FRET in the time of DPSS
> > > >>>
> > > >>> *****
> > > >>> To join, leave or search the confocal microscopy listserv, go
> to:
> > > >>>
> > > > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> > http://lists.umn
> > > > .edu/cgi-bin/wa?A0=confocalmicroscopy>
> > > >>> *****
> > > >>>
> > > >>> Hello all,
> > > >>>
> > > >>> We want to spec a four-laser launch for a new live cell system
> that
> > > > will
> > > >>> handle both CFP/YFP FRET and red/green imaging. However, I am
> sad
> > > > to
> > > >>> see that gas lasers are no longer speccable and so the freebie
> 514
> > > > laser
> > > >>> line is gone. We would therefore have to spec a 514 DPSS and
> forego
> > > > the
> > > >>> far-red line.
> > > >>>
> > > >>> I was wondering whether there is a way to do more (or at least
> the
> > > > same)
> > > >>> with less. 488 nm excites YFP well enough, so in theory I could
> > > > image
> > > >>> CFP/YFP using a scan head dichroic with cutouts for 442 and 488
> nm
> > > > laser
> > > >>> lines. In my experience 442 nm laser excitation (via TIRF)
> causes
> > > >>> negligible YFP excitation and 488 nm does not excite CFP, so it
> is
> > > >>> possible that I could gain speed by passing everything through a
> > > > single
> > > >>> broad bandpass filter (e.g., 455-550 nm) and alternate
> excitations.
> > > >>> Assuming that cross-talk is not a problem, the most significant
> cost
> > > >>> would be that I lose a decent chunk of CFP emission to the scan
> head
> > > >>> dichroic, but in return I gain a 641 nm laser.
> > > >>>
> > > >>> Has anyone tried this? Any feedback on or off-list would be
> much
> > > >>> appreciated.
> > > >>>
> > > >>> Thanks and all the best,
> > > >>>
> > > >>>
> > > >>> TF
> > > >>>
> > > >>> Timothy Feinstein, PhD
> > > >>> Postdoctoral Fellow
> > > >>> Laboratory for GPCR Biology
> > > >>> Dept. of Pharmacology& Chemical Biology
> > > >>>
> > > >>> University of Pittsburgh, School of Medicine
> > > >>> BST W1301, 200 Lothrop St.
> > > >>> Pittsburgh, PA 15261
> > > >>>
> > > >>> -----
> > > >>> No virus found in this message.
> > > >>> Checked by AVG - www.avg.com
> > > >>> Version: 10.0.1411 / Virus Database: 2092/4005 - Release Date:
> > > > 11/08/11
> > > >>>
> > > >>
> > > >> --
> > > >> Damir Sudar - Staff Scientist and Deputy for Technology
> > > >> Lawrence Berkeley Laboratory / Life Sciences Division
> > > >> One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
> > > >> T: 510/486-5346 - F: 510/486-5586 - E: [log in to unmask]
> > > >> WWW: http://www.lbl.gov/lsd/People_**&_Organization/Scientific_**
> > > >>
> > > > Staff_Directory/Sudar_Lab.html<
> > http://www.lbl.gov/lsd/People_&_Organizat
> > > > ion/Scientific_Staff_Directory/Sudar_Lab.html>
> > > >>
> > > >
> > > > -----
> > > > No virus found in this message.
> > > > Checked by AVG - www.avg.com
> > > > Version: 10.0.1411 / Virus Database: 2092/4006 - Release Date:
> 11/09/11
> > >
> >
> >
>
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