*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Antonio,

For this purpose we use dental cement (Picodent TwinSil 22;
http://www.picodent.de/), which is not only biologically inert, though also
can be easily removed after imaging to save the sample for post-imaging
processing.

Josef


On Thu, 27 Feb 2014 15:52:37 +0000, Antonio J Pereira <[log in to unmask]>
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
> 
>Hi all,
>
>For some purposes in live cell imaging we assemble a chamber with a
coverslip on top of a slide.
>We use two halves of another coverslip to act as spacers.
>
>The  issue is the sealing. We've been using VALAP (a kind of wax) that we 
melt and then use to seal all the way through the periphery of the  coverslip.
>
>It works, but it would be more convenient to have something that does not
have to be heated, and that would harden just by  contact with air.
>A bit like the nail varnish used for fixed material ... but here we need
something which is biologically inert.
>
>Any suggestions? Thanks a lot.
>
>Antonio
>
>
>
>António Pereira
>Chromosome Instability and Dynamics Group, Maiato lab
>
>IBMC - Institute for Molecular and Cell Biology
>Porto- Portugal
>
>
>António Pereira
>IBMC - Instituto de Biologia Molecular e Celular
>Grupo Instabilidade e Dinâmica Cromossómica
>
>Rua do Campo Alegre, 823
>4150-180 Porto
>Portugal
>
>+351 22 607 49 00 (ext# 1620)
>[log in to unmask]