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"Porter, Jason" <[log in to unmask]> writes:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=
> confocal
>
> Hi,
>
> I wonder if anyone has tried doing DIC in reflectance (as opposed to
> transmission) or know of any references that might discuss this? I have an
> imaging situation that prohibits cellular imaging in transmission and would
> like to know if it’s possible to use DIC imaging in reflection? Thanks for any
> comments you may have.

If you have some reflecting surface underneath your cells this should
be possible. A quick think about this suggests that you would also need
a 1/4 wave-plate in-front of your DIC prism to get the correct phase
change and decent contrast (although I may have missed something
obvious). 

Ian