In message  <v01540b06af49a6c008d5@[152.1.175.49]> Confocal Microscopy List
writes:
> Dear Jody:  Why should there be a problem with the larger coverslips?
> Generally, you should use a #1 1/2 coverslip since the objectives are
> corrected for that.  Nina Allen

Sometimes a thinner coverslip can be advantageous, if there's a lot of mounting
medium on top of a section of tissue.  The use of a #1-1/2 coverslip assumes
that there's nothing intervening between the bottom of the coverslip and the
sample.  Thus for cells grown on a coverslip, a #1-1/2 is probably perfect.
However, for other applications a #1 may be better.

Martin Wessendorf