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Russell,

Thank you for explaining the functionality of the Leica ROI.  I guess I
should have investigated further before making my assumption.  My apologies!

J

Russell McConnell wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi All,
> The Leica software does have an ROI scanning functionality, the only
> problem is that the button(s) that control it are usually not present on
> the default GUI (graphic user interface).  Go to Tools-->Customize.
> This opens a window with a list of different commands.  Select 'Basic
> Scan' from the list and a number of buttons should appear in the right
> part of the window.  The two that you want are the 'ROI' and 'Config'
> buttons, just drag them onto the main GUI where you want them to be.
> The 'ROI' button lets you define the areas you want to scan, while the
> 'Config' button lets you specify which laser lines you want to use in
> each area (i.e. in ROI-1 you want to use 488 and 568, while in ROI-2 you
> want only 488 etc.).
> This feature is very useful for not only FRAP and FRET analyses, but
> also for checking to see if using a particular laser power is bleaching
> a sample; the AOTF blanks the laser light outside of the ROI so those
> parts of the sample are not exposed to the excitation light.  Any
> confocal with an AOTF should be able to do this type of scanning.
> Cheers,
> Russell
>
> Jill Pflugheber wrote:
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hi John,
>>
>>Having used both systems, I know EXACTLY what you mean.  I have not
>>figured a way to use ROI scanning on the Leica as it is on the Zeiss.
>>For those of you not understanding the Zeiss system, it allows the user
>>to define a ROI to scan using a rectangle, oval, or draw-your-own.  I
>>used that function, for instance, to collect a stack of images of one
>>cell in a field.  I have not found any way to do the same with the
>>Leica--only ROI for analysis.
>>
>>J
>>
>>andrea manazza wrote:
>>
>>
>>>Search the CONFOCAL archive at
>>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>>Hi John. I'm not quite sure I understood your question; anyhow, if I get
>>>the point, the ROI on the SP2 might be used both for analysis and for
>>>scanning (selective bleaching for FRAP, eg).
>>>
>>>I hope this is what you were looking for.
>>>
>>>Best wishes.
>>>
>>>Andrew
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>With the Zeiss confocal systems, it is posible to scan only within a
>>>Region Of Interest.  Is this posible with the Leica SP2 software or is
>>>the Leica ROI only intended for analysis?
>>>
>>>Thank you.  John.
>>>
>>>
>>>
>>>dott. Andrea Manazza
>>>Dipartimento di Oncologia, Università di Torino
>>>CeRMS, ospedale Molinette
>>>Via Santena, 7 - 10126 Torino, Italia
>>>lab: +39-11-6336859
>>>fax: +39-11-6336887
>>>
>
>
> --
> Russell McConnell
> Manager, Tufts-NEMC Imaging Facility
> Department of Neuroscience
> http://www.neurosci.tufts.edu/Imaging
>