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I've been using Invitrogen's 'fluospheres' but they are too bright for my
application (STORM super-resolution microscopy). I'm thinking about making
my own so that I can choose which dyes to use and can titrate down the
amount of dye. Alternatively I could deliberately bleach the Invitrogen
fluospheres I guess.

Can anyone point me in the direction of protocols for:

(1) Staining ~200 nm beads with dyes 
(2) Bleaching a large aliquot of beads in a relatively uniform way

Thanks,

Dan Metcalf,
 
National Physical Laboratory
Teddington
UK


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