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Mycoplasma?

Dan

On Oct 4, 2014, at 8:45 AM, Sunando <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Dear Confocal list users
>  we are seeing a large number of small rod like staining in the dapi channel in ATCC MDA-MB 231 cells. The media and the cells do not turn turbid if incubated at 37Deg C for 48 Hrs.
> 
> We see more of these particles when the cells are grown in absence of PS. In presence of PS, the number grows with passage of cells and actin starts showing very thin needle like outward projections although the cells grow (divide) normally.
> 
> What would be the possible contamination and what is the most probable source and remedy?
> 
> Thank you in advance
> 
> Best
> Sunando
> 
> 
> Sunando Datta
> Assistant Professor
> Dept. of Biological Sciences
> Indian Institute of Science Education and Research
> Bhopal
> India