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Dear confocalists,

there is a study investigating effects of clearing agents on
three-dimensional reconstruction from confocal images which may be of interest.

Bucher, D. et al., Journal of Neuroscience. Methods 100 (2000), 135-143.

Guenter

At 01:04 18.10.02, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We are working on a project to bottle preserve specimens of small animals
>for exhibition purposes: such as small frogs, worms, small fish or small
>animal body organs. We intend to use glycerin for final preservation
>solutions.
>
>There are such commercially prepared specimens which have the soft tissue
>'cleared': meaning that the bodies are semi transparent.
>If the veins and arteries have been injected with color latex then the color
>shows thru.
>
>How is the clearing done?
>
>What are sources for the colored injection materials?
>
>regards
>MRK

------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility
Dept. of Biomedical Optics, MPI fuer Medizinische Forschung
Jahnstr. 29, D-69120 Heidelberg, Germany
Phone (+49) 6221-486-360 (Fax: -325)
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http://sun0.mpimf-heidelberg.mpg.de/~ggiese/lightmicro/index.html