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Hi Milton,

there is no fixed trigger delay in the Bio-Rad MRC600 (and 1000). The
delay between the arrival of an external trigger signal and the actual
start of the line scan is in fact quite variable (varies within several
100 milliseconds).

What we ended up using for synchronization of our voltage clamp setup is
the signal coming from the MRC600 to control the shutter opening. So our
software sends a trigger to the MRC600 and then waits for the shutter
opening signal, and then continues. In addition, we have a custom made
electronic device which, after the shutter-opening occurred, counts down
a user specified number of scan lines and pixels. This allows
synchronization with a given pixel and with microsecond precision, which
is rarely needed.

I hope this helps.

With best wishes


Ernst

Ernst Niggli
Department of Physiology
University of Bern
Buehlplatz 5
CH-3012 Bern / Switzerland

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WWW:
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Fax:   +41 31 631 4611

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Does anyone have a diagram showing the relationship between arrival of a
>pulse on the external input to start linescanning (using yset collect 1 :
>extern command) and the waveforms on the four outputs of a Biorad600?  We
>want to synchronize collection of linescans with a nerve stimulator. I would
>like to trigger the stimulator with one of the Biorad outputs.
>
>Has anyone calibrated the repetition frequency of line scans ie total time
>between the start of one line and the start of the next line in linescanning
>mode?
>
>Thanks
>
>Milton P. Charlton, Professor
>Physiology Department
>Medical Science Building RM.# 3308
>University of Toronto
>1 King's College Circle
>Toronto, ON, M5S1A8
>Canada
>tel:  416-978-6355
>fax:  416-978-4940
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