Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Ernst,
Thank you for your advice on synchronization with a Biorad.  Could you send
the details of that counter?  I guess it looks at the scan output line and
counts that.  Hmm it must also lock out more counting during a frame.
Thank you for sending your line macros to my postdoc Lori.
We have been having a lot of problems with simple line scanning macros
giving errors on our Biorad 600.  Then it turned out that she was using SOM
instead of MPL.  I hope that was the only problem.
Thanks again.
Milt

Milton P. Charlton, Professor
Physiology Department
Medical Science Building RM.# 3308
University of Toronto
1 King's College Circle
Toronto, ON, M5S1A8
Canada
tel:  416-978-6355
fax:  416-978-4940
[log in to unmask]



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Ernst Niggli
Sent: Friday, October 24, 2003 2:12 AM
To: [log in to unmask]
Subject: Re: Output signals on Biorad600, line scan calibration


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Milton,

there is no fixed trigger delay in the Bio-Rad MRC600 (and 1000). The delay
between the arrival of an external trigger signal and the actual start of
the line scan is in fact quite variable (varies within several 100
milliseconds).

What we ended up using for synchronization of our voltage clamp setup is the
signal coming from the MRC600 to control the shutter opening. So our
software sends a trigger to the MRC600 and then waits for the shutter
opening signal, and then continues. In addition, we have a custom made
electronic device which, after the shutter-opening occurred, counts down a
user specified number of scan lines and pixels. This allows synchronization
with a given pixel and with microsecond precision, which is rarely needed.

I hope this helps.

With best wishes


Ernst

Ernst Niggli
Department of Physiology
University of Bern
Buehlplatz 5
CH-3012 Bern / Switzerland

E-mail: [log in to unmask]

WWW:
http://beam.to/Ernst_Niggli
http://beam.to/calcium_quark

Phone: +41 31 631 8730
Fax:   +41 31 631 4611

>Search the CONFOCAL archive at 
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Does anyone have a diagram showing the relationship between arrival of 
>a pulse on the external input to start linescanning (using yset collect 
>1 : extern command) and the waveforms on the four outputs of a 
>Biorad600?  We want to synchronize collection of linescans with a nerve 
>stimulator. I would like to trigger the stimulator with one of the 
>Biorad outputs.
>
>Has anyone calibrated the repetition frequency of line scans ie total 
>time between the start of one line and the start of the next line in 
>linescanning mode?
>
>Thanks
>
>Milton P. Charlton, Professor
>Physiology Department
>Medical Science Building RM.# 3308
>University of Toronto
>1 King's College Circle
>Toronto, ON, M5S1A8
>Canada
>tel:  416-978-6355
>fax:  416-978-4940
>[log in to unmask]