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| Date: | Wed, 11 May 2005 17:45:49 -0700 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Try direct email to either Rachel Errington ([log in to unmask]
<mailto:[log in to unmask]> ) or Paul Smith ([log in to unmask])
<mailto:[log in to unmask])> , or both.
-----Original Message-----
From: simon watkins [mailto:[log in to unmask]]
Sent: Wednesday, May 11, 2005 1:42 PM
To: [log in to unmask]
Subject: Re: DRAQ-5 and tissue sections
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Ok clare...and everyone else
I love Drac 5
We use it at 1:2000 dilution (no more concentrated!), and
mix it with the
secondary antibody (it takes about 45 minutes to give very
clean very
distinct nuclear staining at this dilution). We mount in
aqueous mountants,
(gelvatol). Its very bright and beam stable (we don't use
antifades at
all)..... the only problem is we don't seem to be able to
get in touch with
the company that makes it right now... does anyone else have
this problem?
Simon
Simon C. Watkins Ph.D. FRC Path.
Professor, Cell Biology and Physiology and Immunology
Director Graduate Program in Cell Biology and Physiology
Vice Chair, Cell Biology and Physiology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-2797
URL: http://www.cbi.pitt.edu
-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]] On
Behalf Of Clare Waterman
Sent: Wednesday, May 11, 2005 4:14 PM
To: [log in to unmask]
Subject: Re: DRAQ-5 and tissue sections
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Ask simon Watkins at pitt. He was discussing this recently
but I forget wha
he said
Clare M. Waterman-Storer, Ph.D.
Associate Professor
Laboratory for Cell Motility Studies
Department of Cell Biology CB163
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla CA, 92037 USA
Office: 858-784-9764
Lab: 858-784-9243
Secretary (Lorraine Lathrop): 858-784-9964
Fax: 858-784-9779
Email: [log in to unmask]
-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]] On
Behalf Of Maher, Robert
Sent: Wednesday, May 11, 2005 11:30 AM
To: [log in to unmask]
Subject: DRAQ-5 and tissue sections
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Does anyone have any experience with labeling sections with
the nuclear
probe DRAQ-5 (BioStatus Ltd.)? I'm specifically interested
in the mounting
medium chosen. I labeled tissue sections with DRAQ-5 and
mounted the
sections with ProLong Gold (Molecular Probes). After a day
the staining is
significantly decreased. I've also mounted the sections in
water and
glycerin and have had somewhat better success. Any insight
would be
appreciated.
Robert Maher, Scientist
Safety Sciences Michigan-Ann Arbor
Pfizer Global Research and Development
e-mail: [log in to unmask]
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