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Olympus has a new macroscope with fluorescence as well (SVX10/12),  
but we haven't seen it yet.  Like the Leica (which I will probably  
never see since Leica does not have any presence in the area) it is a  
vertical light path and all light goes to the camera when diverted.   
I have seen the Zeiss, and it is very impressive, but throwing away  
half the fluorescence signal plus the parallax problem seems like a  
bad idea to me. A software solution is a possible fix but will never  
be as good.  I will go for the macroscope option over the stereo  
option when the time comes.  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

On Mar 1, 2006, at 12:40 PM, Julian Smith III wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I am told by our Zeiss rep that Zeiss' new software will take care  
> of this problem.  He wasn't sure about the delivery date on the  
> software, however.  Would Zeiss rep like to chime in here?
> Julian
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Maybe I can add a short word of explanation, since I worked on "in- 
>> focus stacking" myself (i.e. merging images from different focal  
>> planes in order to get an overall sharp image of the object).  
>> Doing this with a stereomicroscope (such as the MZ16FA) is  
>> generally not a good idea, because of parallax problems. Indeed,  
>> the optical paths to the oculars and the camera are not parallel  
>> to the optical axis of the system, which introduces shifts between  
>> different focal planes. One reason for which I suggested the  
>> MacroFluo was that it uses vertical optical paths and thus it does  
>> not have this limitation.
>>
>> Cedric Vonesch
>> PhD student
>> Biomedical Imaging Group
>> EPFL-STI-IOA-LIB
>> BM 4.138 (Batiment BM)
>> Station 17
>> CH-1015 Lausanne
>> Switzerland
>>
>> Phone: +41 (0)21 693 51 36
>> Fax: +41 (0)21 693 37 01
>>
>>
>>
>> Diana Kittleson wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>>  Hello,
>>> I am evaluating macroscopes capable of in-focus stacking for bright
>>> field and fluorescence applications.  I have looked at the  
>>> Olympus MVX10
>>> and the Leica MZ16FA.  The demo Olympus instrument did not have a
>>> z-motor which makes for a difficult comparison.  I would sincerely
>>> appreciate any comments on your satisfaction or recommendations  
>>> for such
>>> a purchase.
>>> Thank you kindly,
>>> Diana
>>>
>>> Diana Kittleson
>>> Strategic Technology
>>> General Mills Riverside Technology
>>> 330 University Avenue Southeast
>>> Minneapolis, Minnesota 55414
>>> 763-764-2330
>>> 763-764-2331 fax
>>>
>>>
>
>
> -- 
> Julian P.S. Smith III
> Dept. of Biology
> Winthrop University
> Rock Hill, SC  29733
> 803-323-2111 x6427 (vox)
> 803-323-3448 (fax)
> 803-524-2347 (cell)