Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

One simple thing is to mount them in very little liquid so they can't swim
about - I've done this with FP-tagged bacteria.  Having them stuck to coated
slides as well is a good way to go too, as suggested by others.

cheers,
Rosemary

Dr Rosemary White               [log in to unmask]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     61 (0)2-6246 5334
Canberra, ACT 2601 
Australia


> From: holly snyder <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Date: Wed, 13 Jun 2007 14:34:03 -0700
> To: [log in to unmask]
> Subject: immobilizing bacteria
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi everyone,
> 
> I am imaging bacteria labelled with GFP and YFP.  My
> questions is how do you immobilize the bacteria so you
> don't end up with a blurred image?  I have heard of
> using formaldehyde but am unfamiliar with this
> procedure. Thanks
> 
> Holly Snyder
> 
> 
>      
> ______________________________________________________________________________
> ______
> Yahoo! oneSearch: Finally, mobile search
> that gives answers, not web links.
> http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC
>