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Christophe

I've read that cascade blue excited with 405 laser is as bright as 
Alexa488, that might be a good option. I don't have personal experience 
with it so can't tell if it fades faster than any other dye.

Lenaldo


Christophe Leterrier wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list,
>
> I would like to use all four lines of our new confocal for 
> immunolabeling (405,488,532,633). For the moment I use 
> Hoechst/Alexa488/Alexa555/Cy5, but I would like to have a fourth 
> antibody labeling using the 405 channel instead of Hoechst. What 
> secondary fluorescent antibody would you recommend ? Is there an 
> Alexa405 or something similar that would be bright and stable ?
>
> Thanks a lot,
>
> Christophe Leterrier
>
> Postdoc
> INSERM UMR641 Neurobiology of ionic channels
> IFR Jean Roche - Mediterranee University
> Marseille, France