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| Date: | Fri, 7 Mar 2008 00:09:15 +0900 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Christophe,
I was also looking for a fluorescent dye for 405 nm excitation, and I compared Alexa 405
with Pacific Blue. Both dyes bleached to some extent with ProlongGold, but Pacific Blue was
absolutely brighter than Alexa 405, when compared at the same dilution. So, I recommend
Pacific Blue, although Molecular Probes sells Pacific Blue for flow cytometry.
I found another problem on Alexa 405: it nonspecifically binds to nuclei ! I asked Molecular
Probes/Invitrogen about this problem, and they recommended me to use their blocking
solution, Image-iT FX Signal Enhancer. It actually worked well, but Pacific Blue does not have
such a problem, so that is another reason I like Pacific Blue.
Junya
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