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Hi Jen and John,

I did try SimFCS a couple of years ago. Limited to 256x256 pixels, which 
defaults to the top left corner (can be adjusted). Good to know that the 
fee goes to a useful cause.

256 pixels at 25 nm pixel size is not much of a field of view.

Sincerely,

George


On 7/12/2011 1:56 AM, Jen Clarke wrote:
> Hi George
>
> Enrico Gratton's "SimFCS" software does N&B analysis
> They used to and probably still do do a 30 day free trial version, and the full version is sold at a very affordable price (was ~$2000 USD when we bought it 2 years ago) with funds raised going towards scholarships for people going to the Laboratory for Fluorescence Dynamics (LFD) for training.
>
> You should be able to find info on the software and lots of other fantastic bits of info at the LFD website
>
> Kind regards
> Jen
> --
> Jennifer Clarke BSc (Hons) PhD
> Research Associate, Anatomy and Histology
> Centre for Neuroscience, School of Medicine
> &
> Facility Manager, Optical Microscopy Suite, Flinders Microscopy
> (training and assistance available on Mondays only)
>
> Flinders University
> GPO Box 2100, Adelaide 5001
> Phone: 61 8 8204 6454/ 61 8 8204 6637
> Email: [log in to unmask]
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] On Behalf Of George McNamara [[log in to unmask]]
> Sent: Tuesday, 12 July 2011 12:34 PM
> To: [log in to unmask]
> Subject: ImageJ plugin for Number and Molecular brightness (N&B) Method?
>
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Dear Confocal listserv,
>
> If anyone is aware of an ImageJ plugin for Number and Molecular
> brightness (N&B) Method, could you please post the link here?
>
> An explanation of N&B can be found in
> http://www.lfd.uci.edu/workshop/2010/files/LFDWorkshop2010-Lecture05.pdf
> as well as various papers by Digman, Gratton et al.  (page 33 of the pdf
> file).
>
> If there is one, could someone please write one in time for me to use
> for a CW-STED demo Aug 22-Sept 6?
>
> If you are feeling ambitious, Cross N&B would be great to have, too. The
> pdf is not explicit, but usually 100 images are acquired. Figure on
> calculating from a stack in ImageJ (two stacks for cross). I'll be happy
> to send you nice data to use in your publication announcing your plugin.
> You are welcome to visit Miami during the demo to acquire however you
> think is best - however, this is the rainy season (and I have no travel
> budget). I anticipate using the demo instrument's HyD detector in photon
> counting mode for most of this.
>
>
> thanks,
>
> George
>
>
> --
>
>
> George McNamara, PhD
> Analytical Imaging Core Facility
> University of Miami
>
>    


-- 


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami