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Dear George,
I don't know if anyone has an image J plugin for the N&B analysis. However
Jay Unruh from Stowers has a collection of image J plugins for FCS,
CCRICS, RICS and PCH. Here is a link to those plugins:
http://research.stowers.org/imagejplugins/zipped_plugins.html
The SIMFCS software can be used for N&B analysis. If you are interested
you can download the software from: http://www.lfd.uci.edu/globals/
We can send you tutorials on how to use the program for the N&B analysis.
Kind regards,
Michelle
*******************************
Michelle Digman, Ph.D.
University of California, Irvine
Laboratory for Fluorescence Dynamics
3204 Natural Sciences II
(949) 824-2992
http://www.lfd.uci.edu/
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Confocal listserv,
>
> If anyone is aware of an ImageJ plugin for Number and Molecular
> brightness (N&B) Method, could you please post the link here?
>
> An explanation of N&B can be found in
> http://www.lfd.uci.edu/workshop/2010/files/LFDWorkshop2010-Lecture05.pdf
> as well as various papers by Digman, Gratton et al. (page 33 of the pdf
> file).
>
> If there is one, could someone please write one in time for me to use
> for a CW-STED demo Aug 22-Sept 6?
>
> If you are feeling ambitious, Cross N&B would be great to have, too. The
> pdf is not explicit, but usually 100 images are acquired. Figure on
> calculating from a stack in ImageJ (two stacks for cross). I'll be happy
> to send you nice data to use in your publication announcing your plugin.
> You are welcome to visit Miami during the demo to acquire however you
> think is best - however, this is the rainy season (and I have no travel
> budget). I anticipate using the demo instrument's HyD detector in photon
> counting mode for most of this.
>
>
> thanks,
>
> George
>
>
> --
>
>
> George McNamara, PhD
> Analytical Imaging Core Facility
> University of Miami
>
>
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