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November 2011

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From:
Kurt Thorn <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 4 Nov 2011 13:15:04 -0700
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*****
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Dyomics has some large stokes shift dyes that absorb in the green and 
emit in the red/far red.  We looked into them a while ago for this 
purpose but never had a user who was sufficiently motivated to test 
them, so I don't know how well they work.  See 
http://www.dyomics.com/dy-480xl.html?&L=1%2F%2Findex.php%3Foption%3Dcom_fabrik

I think there may be another company with large stokes shift dyes, but I 
can't remember who.

Kurt

On 11/4/2011 9:36 AM, Simon Walker wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
> Has anyone successfully imaged 5 colours in the same sample with their
> confocal?  We have a user who is keen to do this.  They are already using
> DAPI, AF488, AF555&  AF647 so if there's something that maybe excites in the
> blue and emits in the red/far red?  Or if that's not possible a completely
> different set of 5.  We have the usual range of excitation wavelengths from
> 405 up to 635 nm.
> Thanks,
> Simon
>

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