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Dyomics has some large stokes shift dyes that absorb in the green and
emit in the red/far red. We looked into them a while ago for this
purpose but never had a user who was sufficiently motivated to test
them, so I don't know how well they work. See
http://www.dyomics.com/dy-480xl.html?&L=1%2F%2Findex.php%3Foption%3Dcom_fabrik
I think there may be another company with large stokes shift dyes, but I
can't remember who.
Kurt
On 11/4/2011 9:36 AM, Simon Walker wrote:
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>
> Hello,
> Has anyone successfully imaged 5 colours in the same sample with their
> confocal? We have a user who is keen to do this. They are already using
> DAPI, AF488, AF555& AF647 so if there's something that maybe excites in the
> blue and emits in the red/far red? Or if that's not possible a completely
> different set of 5. We have the usual range of excitation wavelengths from
> 405 up to 635 nm.
> Thanks,
> Simon
>