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In the 'Advanced Information' from the Nobel Committee they actually put the publications in bold which were key for the award, in the case of WE Moerner these are:
Moerner WE and Kador L (1989) Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett.62:2535-2538.
Dickson RM, Cubitt AB, Tsien RY and Moerner WE (1997) On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388:355-358.
So it is quite clear, the first detection of single molecules in a solid and the blinking behaviour of GFP, which links it quite nicely to Eric Betzig's work. In Betzig's case the early NSMO work is not in bold, but the 'Proposed Method' paper from 1995 is, putting him ahead of others in the field. Sometimes it is good to publish an idea on its own.
Anyhow, i think with the effort of the whole community including funding organisations, journal editors and countless students, this Nobel prize would not have been possible, well done everyone! Let's not forget, there were only a few microscope techniques which were worth a nobel prize before: Zsigmondi (ultramicroscope), Zernike (phase contrast), Ruska (Electronmicroscope, Binning & Rohrer (scanning tunnelling microscope).
best wishes
Andreas
-----Original Message-----
From: Andrew York <[log in to unmask]>
To: CONFOCALMICROSCOPY <[log in to unmask]>
Sent: Fri, 10 Oct 2014 13:19
Subject: Re: congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry, for the development of super-resolved fluorescence microscopy
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I think the list will find this article entertaining:
http://www.slate.com/articles/health_and_science/science/2014/10/chemistry_nobel_prize_winners_see_microscope_images_they_made_possible_photos.html
Pay particular attention to the images.
Unrelated: I assume Betzig's award is primarily due to his demonstration of
PALM, and related follow-up work. I assume Hell's is similarly for his
demonstration of STED. What work of Moerner's are they thinking of? Is
there one specific paper/idea, like with Betzig and Hell, or is it for
decades of work on single molecules in general?
On Oct 10, 2014 4:25 AM, "Andreas Schönle" <
[log in to unmask]> wrote:
> *****
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> *****
>
> Dear Guy, Nuno, dear list members!
>
> Surely this discussion will now go on for a while but I would like to set a
> few facts straight, so it can be put on solid ground.
>
> 1)
> Stefan Hell was never a student of Chirstoph Cremer. His PhD advisor was
> Prof. S. Hunklinger and both 4pi and STED microscopy were conceived by him
> independently. The principle of STED is completely unrelated to 4pi and 4pi
> has nothing to do with what the Nobel prize was awarded for (see below).
> 2)
> 4pi microscopy is not a super-resolution method (it is diffraction
> limited).
> Christoph Cremer's original proposal to use light coming from all
> directions
> was aimed at increasing lateral resolution and was based on the wrong
> assumption that inverting the light-field of a point source <<lambda by
> means of a hologram could beat the diffraction limit.
> (this neglects the fact that the near field decays exponentially and that
> this cannot be inverted in conventional materials)
> 3)
> Localization is not resolution. Impressive work was done localizing single
> or several light sources separable by spectral properties, stepwise
> bleaching etc. - usually with the goal of measuring distances. But dense
> images can (as of now) only be obtained by time-sequentially switching
> molecules on and off.
> This was first realized for ensembles in STED and molecule-by-molecule in
> PALM/STORM/FPALM.
>
> Again, this is not to diminish anybody's work in the field. Over the years
> I
> have read many articles describing important and inspiring work. And I
> wholeheartedly agree that the field would not be where it is without these
> contributions.
> But when discussing who should be honored for finding the key to circumvent
> the diffraction limit in optical imaging, it is important to identify those
> people that had the right idea, recognized its importance and proved this
> by
> actually putting it to work.
>
> Best regards,
> Andreas
>
> --
> Andreas Schoenle, Dr.
> Abberior Instruments GmbH, D-37077 Goettingen, Germany
>
> phone: +49 (551) 30724170
> fax: +49 (551) 30724171
> http://www.abberior-instruments.com
> mailto:[log in to unmask]
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On
> Behalf Of Nuno Moreno
> Sent: Freitag, 10. Oktober 2014 00:19
> To: [log in to unmask]
> Subject: Re: congratulations to Eric, Stefan and W . E. for Nob el P rize
> in
> Chemistry, ³ for t he development of super-reso lved fluores cenc e mi
> croscopy²
>
> *****
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> *****
>
> Dear Guy
>
> You're absolutely right. I actually sent an internal email (for the
> institute) a few hours after the announcement with, among other stuff,
> this:
>
> "As always there are many others that should be on the laureates list but
> one needed the final click. In my opinion Cremer from Heidelberg university
> should be one of them ...."
>
> Nuno Moreno
>
>
>
>
>
> On 09 Oct 2014, at 02:16, Guy Cox <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > The three names is always a problem - especially when the prize is
> awarded
> essentially for two different techniques. Christoph Cremer (Stefan's
> supervisor) could well also feel he'd been passed over.
> >
> > Guy
> >
>
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