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January 2015

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Subject:
Re: YFP/Venus and LED excitation sources
From:
Craig Brideau <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 14 Jan 2015 12:56:43 -0700
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Sounds like a technical issue. 470nm should work well for YFP. It's either
a filter problem or the fluorophore was a dud.

Craig


On Wed, Jan 14, 2015 at 10:50 AM, Aryeh Weiss <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> A colleague  of mine told me that he is unable to see YFP/Venus with his
> LED source (Lumencore, 470nm LED). This surprises me, even if 470nm is not
> at the excitation peak of YFP.
> Are there list-members who  use LED excitation sources with YFP? If so, I
> would appreciate if they would share their experience with me.
>
> Thanks in advance.
> --aryeh
>

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