Hi everyone,
We are immunostaining for
macrophages and dendritic cells.
and have encountered what appears to be endogenous biotin as avidin
conjugates alone seemed to stain tissue.  Has anyone encountered this
problem before and are they aware of
any techniques that block endogenous biotin in tissue prior to staining?
Look forward to your replies.
 
Natalia Bilyk
Dept Anatomy and Human Biology
University of Western Australia
 email [log in to unmask]