CONFOCALMICROSCOPY Archives

May 1995

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Subject:
Re: Endogenous biotin
From:
Karl Bellve <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 23 May 1995 09:54:54 EST
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Hi Margaret (Margaret Fransen), in <[log in to unmask]> on May 23 you said
 
> Regarding possible endogenous ligands for streptavidin, there was a report
> in 1994 (or 93) that streptavidin bound to integrins.  Perhaps that could
> account for some "non-specific" labelling with biotin/avidin techniques.
> Margaret Fransen, Ph.D.
> Dept. of Anatomy & Cell Biology
> SUNY Health Science Center @ Syracuse
> 750 E. Adams St. Syracuse NY 13210
> Tel: 315 464 8525
> Fax: 315 464 8535
> E-mail: [log in to unmask]
>
>
 
This is TRUE, also plain avidin has alot of sugar groups on its surface.
I use Neutralite Avidin from Molecular Probes. It has its sugar groups
striped.
 
 
 
Karl Bellve                             LLLL  L     L LLLLL  LLLL    LLLL
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