CONFOCALMICROSCOPY Archives

March 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: mountant for lipid probes
From:
"Dr. Gordon Warren" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sat, 8 Mar 1997 10:56:55 -0600
Content-Type:
text/plain
Parts/Attachments:
text/plain (23 lines) 
> Subject: mountant for lipid probes
> Could someone recommend an anti-fade mountant for use with DiI/DiO labelled
> cells.  The usual glycerol-based mounting media cannot be used because the
> dyes "bleed." Molecular Probes recommended something called Cytoseal.  Has
> anyone tried it?  Any other suggestions?

        I recall no problems when using glycerin jelly with DiOC18(3). Even
though it is glycerol based, it solidifies in 1 hr or less after mounting. You
could add DABCO or whatever to the glycerin jelly but I don't recall much of a
bleaching problem with DiO.


--
Gordon L. Warren, Ph.D.
Research Scientist
Muscle Biology Laboratory
158 Read Building
Texas A&M University
College Station, TX 77843-4243
Fax (409) 862-4808
Phone (409) 862-4809 office
e-mail [log in to unmask]

ATOM RSS1 RSS2