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April 2024

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From:
Glyn Nelson <[log in to unmask]>
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Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 19 Apr 2024 07:54:30 -0500
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Hi Kyle,

It does depend what they are trying to analyse from their data.  We did a comparison of  sub diffraction bead size as measured by Full Width at Half Maximum in xy and z using 23oC oil at different temps (see Fig 2 in here https://dx.doi.org/10.17504/protocols.io.bp2l61ww1vqe/v1 ).  The differences in lateral resolution were slight, but axial is really bad.  So if they are imaging in 2D and consistently at the same depth, they will probably get reasonably good data.  Any volume analysis or object analysis at different depths will suffer though. 

Lastly, the different oils also have different dispersions, and manufacturer's recommend using their oil with their objectives as the dispersion of the oil is matched to their objective design.  You may only see a slight effect depending on how different they are (you can see the Abbe Number on the bottle (measured as Vd or Ve).  Talking of which, does anyone know of a table that correlates the measures at different wavelengths (if that is possible, or too complex depending on the material)?

Glyn

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