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Belt wrenches have saved me multiple times on multiple different stands over the years. 


Best,

Gary

> On Apr 24, 2024, at 2:27 PM, Brian Armstrong <[log in to unmask]> wrote:
> 
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> 
> Hi Mika, for the objective, I have soaked the front lens in 95% ethanol and then worked at it with a cotton swab. I also use the wooden end of the swab stick to push and pry at the stuck oil. I once had a lens that I thought was hopeless and considered xylene, but instead I kept working at it for around 3 days soaking it in ethanol over and over again and eventually it came off when using the wooden end of the swab.
> I imagine that the same theory could be applied to the objective stuck into the stand but that is a tricky matter as you do not want to send any liquids down into the stand.
> 
> Best of luck,
> 
> Brian Armstrong PhD
> Research Professor
> Director, Light Microscopy Core
> Beckman Research Institute
> City of Hope
> 
> From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Mika Ruonala
> Sent: Wednesday, April 24, 2024 10:37 AM
> To: [log in to unmask]
> Subject: Hardened immersion oil
> 
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> 
> Dear all,
> 
> I have today encountered a very unnerving phenomena on a Nikon microscope. The system has not been used for over a year and then also only for fixed specimen. During inspection I noticed that. It only the stage has some clear spills that are hard as glass but also two oil immersion objectives have similar hard crystals. For the 60x oil lens there is a run of the material alongside the lense towards the revolver, and the spring of a 40x oil immersion lense seems to be completely stuck, this lense also cannot be removed from the revolver, it’s like glued on the mount.
> 
> 
> 
> No live imaging what so ever was done on the system and the user has only used Nikon type F immersion oil. My fear is this is not properly cleaned immersion oil. Has anyone seen something like this before, and if so, how can all be cleaned?
> 
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