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Thanks Phil,

that sounds really good, also for custom organ-on-chip approaches.
Quick question: you kept the fern alive, I guess at room temperature.
Have you /any tried it at 37°C in an incubator?

Thanks

Axel

On 2024-04-23 00:53, [log in to unmask] wrote:

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> 
> Julia -
> 
> Here's a method that may work for you. It is a well known botanical 
> method that I have used many times without any toxicity problems. It's 
> cheap and easy, and it seals well. It's autoclavable, and it keeps 
> forever un-refrigerated. I have some always on hand. The sealant is 
> called VaLaP and it's cheap and non-toxic. Get yourself some plain 
> Vaseline (unscented etc.) and some ordinary canning paraffin from the 
> supermarket, and some lanolin, which you can get in bulk from VWR or 
> Fisher where they list it as "Wool Fat". Mix them well 1:1:1 (equal 
> parts) at 60C. I have a little hotplate that I keep just for my VaLaP, 
> always ready. When you need it melt it at 60C and apply it with a 
> Q-tip. I dab it on the corners of the coverslip just to keep it in 
> place, and then paint around the edge to seal. It is very sticky so it 
> seals well and will stick to polycarbonate or glass, and yet it is not 
> difficult to handle once you have tried it a couple of times.
> 
> I have kept fern material alive for an extended period for a week or 
> more of continuous time-lapse. I autoclave in a little beaker. No 
> contamination or leakage. Obviously, you don't want to get in on the 
> objective, but If you get it on the lens it cleans off well with 
> "Sparkle".
> 
> Try it. you may find it is just what you need.
> 
> Regards,
> 
> Phil Lintilhac
> 
> ------------------------------------------------------------------------
> On 4/22/24 1:43 PM, Julia Edgar wrote:
> 
>> *****
>> To join or leave the confocal microscopy listserv or to change your 
>> email address, go to:
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>> Post images onhttp://www.imgur.com  and include the link in your 
>> posting.
>> *****
>> 
>> Dear Confocal Microscopy List
>> Years ago I got a very useful protocol from somebody on the List for 
>> making live imaging dishes. Basically, one burred holes in a Petri 
>> dish and glued a suitable coverslip beneath using World Precision 
>> Instruments, #SYLG184. We continue to use Slyfard (R) 184, but we 
>> can't keep the cells alive. It seems like the glue is somehow toxic to 
>> the cells - yet we had great success in the past.
>> 
>> Any advice will be greatly appreciated.
>> Best wishes
>> Julia