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April 2024

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Subject:
Re: EXT: Re: vesicles
From:
"Kilgore, Jason A." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 11 Apr 2024 23:57:20 +0000
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Yes, I believe so, though the signal may be dim compared to the nuclei. (using Hoechst 33342 or Hoechst 33258).

Jason

Jason A. Kilgore
Supervisor, Cell Analysis Tech Support
Life Sciences Solutions
Thermo Fisher Scientific

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Model, Michael
Sent: Thursday, April 11, 2024 4:51 PM
To: [log in to unmask]
Subject: Re: EXT: Re: vesicles

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Interesting! If this were so, would Hoechst stain them?

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Kilgore, Jason A.
Sent: Thursday, April 11, 2024 7:44 PM
To: [log in to unmask]
Subject: Re: EXT: Re: vesicles

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Michael,

I hope I'm wrong about this, but it reminds me of images of mycoplasma-infected cells. Those cells also don't look very healthy. Could they be infected?

Jason

Jason A. Kilgore
Supervisor, Cell Analysis Tech Support
Life Sciences Solutions
Thermo Fisher Scientific

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Model, Michael
Sent: Thursday, April 11, 2024 4:38 PM
To: [log in to unmask]
Subject: Re: EXT: Re: vesicles

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Thank you, Tim,
But I don't think this is it. I placed one of the images here:

https://urldefense.com/v3/__https://www.dropbox.com/scl/fo/3p8yfmm5rqoo1rqj36bkd/AJOTl2uNKOh6DSXztwO2HWI?rlkey=h771q3h1abhtssv327dng2yre&dl=0__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFgMjkrAq$

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Tim F
Sent: Thursday, April 11, 2024 5:24 PM
To: [log in to unmask]
Subject: EXT: Re: vesicles

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Hi Michael, just to confirm, if your experience is similar to the video below, do you mean the dark spots? Thanks to Nikon for the vid!

https://urldefense.com/v3/__https://youtu.be/Rb-Q3wLsbIk?si=f8GSrHrkTEYmZg1t__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFk1AOE-7$

I remember thinking those are protein mis-folding aggregates. I don't think you would see endosomes with that kind of contrast, and vesicles are too small at 60nm to see with transmitted light.

Best,


T

Timothy Feinstein, Ph.D.

On Thu, Apr 11, 2024 at 3:53 PM Model, Michael <[log in to unmask]> wrote:

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> *****
>
> Dear List,
>
> If you look at cultured cells, such as HeLa, under bright field, you 
> clearly see many vesicles about 1 um. Does anyone know what they are?
> (They are not acidic). The stupidest thing is that I once found a 
> paper that investigates them, but now I cannot find that paper!
>
> Mike
>
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