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Date: | Mon, 22 Apr 2024 11:20:50 -0700 |
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*****
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*****
Hi Julia,
Considering the cost of the time to make the dishes and find a suitable
glue, it might be worth considering to purchase glass bottom dishes online.
There are a number of companies-you can even get them precoated with things
like polyLysine, matrigel, collagen, etc.
Best,
Chris
On Mon, Apr 22, 2024, 10:44 AM Julia Edgar <[log in to unmask]>
wrote:
> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Confocal Microscopy List
> Years ago I got a very useful protocol from somebody on the List for
> making live imaging dishes. Basically, one burred holes in a Petri dish and
> glued a suitable coverslip beneath using World Precision Instruments,
> #SYLG184. We continue to use Slyfard (R) 184, but we can't keep the cells
> alive. It seems like the glue is somehow toxic to the cells - yet we had
> great success in the past.
>
> Any advice will be greatly appreciated.
> Best wishes
> Julia
>
>
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