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Hi Emmanuel,
It looks that one of the camera is closer to the microscope than the other. Try to check that all the connections are flush and tight. 

Catalin

> On Apr 15, 2024, at 09:30, Emmanuel Levy <[log in to unmask]> wrote:
> 
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> 
> Dear All,
> 
> We have a W1 spinning disk with dual cameras. We recently had them aligned,
> and upon close inspection, we see that the GFP channel image is about 1 to
> 2 pixels larger than the RFP image in every corner (we used a 60x
> objective, and the cameras are primeBSI express with 6.4um pixels, so the
> image is off by 100-200nm in every corner). you can download the two images
> as a composite here if you are curious:
> https://drive.google.com/file/d/1TWIxwiZF6uf3FspyA96AX6r092PB_102/view?usp=sharing
> 
> What would be the best way to solve this issue? Shouldn't the standard
> camera mounts enable us to correct this?
> 
> Thanks for your help and comments,
> Best wishes,
> 
> Emmanuel