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Hi Ben,

This is uncommon on  the slow axis.

I'm not familiar with your CLSM system, but the only things that come to
mind are 1) incorrect galvo linearization - should be solved through
software, though I don't know the procedure as I'm not one of the lucky
owners of the Zeiss calibration lens+target;
2) a plain failure of the galvo amplifier (might be tough to find a
replacement though) - an oscilloscope trace of the input and output will be
most informative.

To differentiate between the two, what's the behavior when you zoom in?

PS, there could be any number of other causes... e.g. the feedback
photodiodes...
Best, zdenek
-- 
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth



On Fri, Mar 29, 2024 at 7:06 PM Benjamin E Smith <
[log in to unmask]> wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hey Microscopists,
>
> We have an odd aberration on an older Zeiss LSCM that I can't quite wrap my
> head around, so I was wondering if anyone else has ever seen this.  In the
> middle of the scan field, the image becomes stretched, and then returns to
> normal towards the end of the scan field (see images below - yellow
> outline):
> https://bit.ly/3TXrfxl
> https://bit.ly/3TXEsq8
>
> At first I was thinking it could be the Y-mirror binding, but usually you
> will see a compression after the stretch when the mirror breaks free, and a
> corresponding jello-like oscillation, neither of which we see here.  It
> also can't be debris on the Y-mirror as this would create a vertical
> distortion, not a horizontal one.  So, I'm stumped.
>
> Any insights or ideas would be greatly appreciated,
>    Ben Smith
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Weill Hall
> Berkeley, CA  94720-3200
> Tel  (510) 642-9712
> Fax (510) 643-6791
> e-mail: [log in to unmask]
>
> https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/
>