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Hi everybody,
In my experience, HyD X detectors start to show comets in the images after being severely overexposed to light. If afterwards one lets them image a standard fluorescent sample for a while (e.g., an hour) the comets gradually disappear and eventually go away completely. Any sample with a significant fluorescent signal can be used for this purpose. I hope that helps.
Best regards,
Sergio Lopez
Dr Sergio G Lopez
Bioimaging Facility
Optical Microscopy Support Specialist
Cell & Developmental Biology
Room G22, Biffen Building
John Innes Centre
01603 450298
________________________________
From: Confocal Microscopy List <[log in to unmask]> on behalf of Jeremy Adler <[log in to unmask]>
Sent: 15 April 2024 10:09
To: [log in to unmask] <[log in to unmask]>
Subject: Re: HyD comet artifact?
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Hi Petro,
Could you explain what the Hyd comet artefact is/was.
Is the fix, software is so which version.
Best
===============================================
B i o V i s P l a t f o r m of Uppsala University
Light & EM microscopy / FlowCytometry & Cell Sorting / Image Analysis
===============================================
Jeremy Adler PhD - Senior research engineer
Light, Confocal Microscopy, Image Analysis
E-mail: [log in to unmask]
0739 188170
Dag Hammarskjölds v 20
751 85 UPPSALA, SWEDEN
http://biovis.uu.se/
===============================================
-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Khoroshyy Petro
Sent: Monday, April 15, 2024 10:53 AM
To: [log in to unmask]
Subject: Re: HyD comet artifact?
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Hi.
Sorry.
It looks like I posted to this list instead of our facility users mailing list.
My apologies.
Petro.
On Mon, 15 Apr 2024, 10:11 Khoroshyy Petro, <[log in to unmask]> wrote:
> Hi everybody.
> The comet artifact is fixed.
> If you have it anytime I future.
> Please let me know.
> Best regards.
> Petro.
>
> On Sun, 14 Apr 2024, 09:20 Khoroshyy Petro, <[log in to unmask]> wrote:
>
>> Hi everybody.
>> Could you educate me about the comet artifact of HyD and why it happens?
>> Is there anything I can do to prevent this from happening?
>> What is the latest information on it?
>> Thanks.
>> Petro.
>>
>> --
>> ______________________________
>> Petro Khoroshyy
>>
>
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