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Hi Kyle,
I recommend type 37 oil from Cargille
https://www.cargille.com/

(no commercial interest)
best,
Guy Hagen

On Fri, Apr 19, 2024 at 9:16 AM Kyle Michael Douglass <
[log in to unmask]> wrote:

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>
> Thank you, Glyn!
>
> For the record, we also contacted Olympus and they said everything is fine
> as long as you use their oil up to 40 deg C, which surprises me a little
> bit given your data and the fact that other manufacturers sell oil
> specifically for use at 37 C.
>
> Cheers,
> Kyle
>
> -----Original Message-----
> From: Confocal Microscopy List <[log in to unmask]> On
> Behalf Of Glyn Nelson
> Sent: Friday, April 19, 2024 14:55
> To: [log in to unmask]
> Subject: Re: 37 deg C Olympus immersion oil
>
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>
> Hi Kyle,
>
> It does depend what they are trying to analyse from their data.  We did a
> comparison of  sub diffraction bead size as measured by Full Width at Half
> Maximum in xy and z using 23oC oil at different temps (see Fig 2 in here
> https://dx.doi.org/10.17504/protocols.io.bp2l61ww1vqe/v1 ).  The
> differences in lateral resolution were slight, but axial is really bad.  So
> if they are imaging in 2D and consistently at the same depth, they will
> probably get reasonably good data.  Any volume analysis or object analysis
> at different depths will suffer though.
>
> Lastly, the different oils also have different dispersions, and
> manufacturer's recommend using their oil with their objectives as the
> dispersion of the oil is matched to their objective design.  You may only
> see a slight effect depending on how different they are (you can see the
> Abbe Number on the bottle (measured as Vd or Ve).  Talking of which, does
> anyone know of a table that correlates the measures at different
> wavelengths (if that is possible, or too complex depending on the material)?
>
> Glyn
>