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I try to clean stubborn dirt first with xylene and, if that doesn't work, with acetone. Needless to say, organic solvents must be applied briefly and in small quantities. For a stuck objective, I would try something like a belt wrench. WD40 might also help loosen the objective, but I don't know how safe it is - don't take my word for it. Good luck!

Mike

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Mika Ruonala
Sent: Wednesday, April 24, 2024 1:37 PM
To: [log in to unmask]
Subject: EXT: Hardened immersion oil

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Dear all,
I have today encountered a very unnerving phenomena on a Nikon microscope. The system has not been used for over a year and then also only for fixed specimen. During inspection I noticed that. It only the stage has some clear spills that are hard as glass but also two oil immersion objectives have similar hard crystals. For the 60x oil lens there is a run of the material alongside the lense towards the revolver, and the spring of a 40x oil immersion lense seems to be completely stuck, this lense also cannot be removed from the revolver, it's like glued on the mount.

No live imaging what so ever was done on the system and the user has only used Nikon type F immersion oil. My fear is this is not properly cleaned immersion oil. Has anyone seen something like this before, and if so, how can all be cleaned?
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